Analizó el efecto de la deglicosilación de los antígenos TES (dTES) en la detección de anticuerpos IgG, IgM e IgE mediante la pruebas de ELISA y Western – Blot. Los resultados muestraron que los antígenos TES y dTES tuvieron una sensibilidad y especificidad del 100% para la detección de anticuerpos IgG, con menor reactividad cruzada para los antígenos dTES (5,3%) en comparación con los antígenos TES (13,3 %). En la prueba de Western – blot, se obtuvo una sensibilidad y especificidad del 100% para ambos antígenos y la reactividad cruzada fue eliminada totalmente cuando se utilizaron los antígenos dTES. La detección de anticuerpos IgM por ELISA mostró reactividad inespecífica, con sensibilidades de 39,7% y 34,5% y especifidades del 95,8% y del 96,8%, para los antígenos TES y dTES, respectivamente, y con ocurrencia de reactividad cruzada de 24% y 28% para ambos antígenos...

Objective. To estimate frequency of toxocariosis in Lima Region. Methods. During 2013, a non-randomized serological survey was performed by Elisa technique in four Lima Region provinces’ (Cajatambo, Canta, Huaral and Yauyos) inhabitants. Sera samples were obtained by DIRESA Lima and Regional Reference Laboratory of Public Health workers and processed at Tropical Medicine Institute “Daniel A. Carrion” of Universidad Nacional Mayor de San Marcos, Lima, Peru. Results. From 442 sera samples obtained 142 (32.1%) were positive to Toxocara; 54% of participants were female, 31% were between 31-59 years, 45% had a dog or cat at home. There were no significant differences between serology and ages or sex or in having dog or cats. Conclusions. Toxocariosis is frequent in Lima Region inhabitants. It is necessary to perform representative studies to determine prevalence of this infection.

ntroduction. Diagnosis of some zoonosis in different provinces from the Lima region, motivated on epidemiological investigation. Objective. To determine the prevalence of hydatidosis (echinococosis) in population from Lima region by ELISA IgE test compared with Western Blot. Methods. 560 sera samples were collected from people living in five provinces of Lima region: Canta (n=180), Huaral (n=55), Yauyos (n=133), Cajatambo (n=32) and Huaura (n=160). Antibodies IgE and IgG were detected by ELISA test and Western Blot respectively. Results. 39 samples were positives to ELISA IgE test (seroprevalence 6,96%) and 33 samples were positives to Western Blot IgG test (seroprevalence 5,89%), with light bands of 8 and 16 kDa. Positive samples proceeded from people of provinces of Canta, Yauyos and Huaura. Concordancy were determined between both tests (kappa index = 0,91). 50% (20/39) of positive su...