The snake´s venoms are endogenous secretions constituted for proteins and can produce different symptomatology and in some cases the dead. Using the electrophoretic technique in polyacrylamide discontinuous gel of 7.5% and 12% whit sodium dodecylsulphate (PAGE-SDS) in reductions conditions were carried on a protein analysis of the venom in 5 Bothrops atrox snake´s from 5 different geographical zones to the Peruvian Amazon using centrifuge crude venom, additionally the proteins were quantification for using an spectrophotometer following the Biuret method. The results evidence the presents of different bands in the proteins, in ranges of 14 kD to 116 kD, finding 9, 11,8,7,8 bands of proteins for the populations of Moena Caño, Isla Iquitos, Panguana, Genaro Herrera and Nina Rumi, while the quantification show the presents to major quantity of protein in the snake from Isla Iquitos (67.4...

Molecular research demand the creation of new protocols and permit the obtain a high quality DNA in the most quantity of species and know the genetic diversity, for that objective of the present research was obtained the purification and amplification of scales DNA in the snake Bothrops atrox. the scales were recollected in the field using the visual encounter survey [5] The For the purification of the DNA was modification the protocol [4], the DNA purity was verifiable for the electrophoretic method and it quality and concentration for spectrophotometry, the amplification was carried for Polymerase chain reaction-PCR using random primers. The results show the successful DNA purification, showing an acceptable quality ratio (2) and concentration (585.85 µg/mL), at the same time was made the amplification of DNA for PCR. We conclude indicating the useful of this technique for the purific...