With the object of find and / or to determine a methodology for the collection and preservation of pollen grains of Sacha inchi (Plukenetia volubilis L.), were taken into account two phonological stages of flowering. The first stage before anthesis and the second stage after anthesis, this in order to know the behavior of both phases of flowering and viability of pollen grains with respect to germination and formation of fruit. The excellent preservation of pollen grains, shows that both the collection and preservation method used in this research work are the most optimal and appropriate. We use one of the most important parameters for temperature (T°), two scales as 8 º C and -8 º C, which responded excellently to the action to conserve the pollen grains, while in a perfect state of dormancy SUS morphological, physiological and genetic, so make use of both methods because it demonst...