The aim of this study was to evaluate the cryopreservation of Brycon henni semen using three programmable freezing curves and two permeable cryoprotectants. Semen of 80 males were diluted in medium supplemented with ethylene glycol (EG) or dimethylsulfoxide (DMSO), and cryopreserved in semen straws using three programmable freezing curves: slow (66 min, -0.42 °C/min), medium (43.3 min, -0.6 °C/min) and ultra-rapid (7.7 min, -5.19 °C/min). After one month of storage, semen was thawed and motility was evaluated through a class analysis system (SCA®) and sperm vitality (EV) by fluorescence microscopy with SYBR14/IP probes. For statistical analysis, generalized linear models (GLM) were adjusted and means compared by the Tukey test. The medium and ultrarapid velocity curves showed higher and equivalent values for total motility, progressive motility, and linear velocity of sperm (p<0.0...

The aim of this study was to evaluate the cryopreservation of Brycon henni semen using three programmable freezing curves and two permeable cryoprotectants. Semen of 80 males were diluted in medium supplemented with ethylene glycol (EG) or dimethylsulfoxide (DMSO), and cryopreserved in semen straws using three programmable freezing curves: slow (66 min, -0.42 °C/min), medium (43.3 min, -0.6 °C/min) and ultra-rapid (7.7 min, -5.19 °C/min). After one month of storage, semen was thawed and motility was evaluated through a class analysis system (SCA®) and sperm vitality (EV) by fluorescence microscopy with SYBR14/IP probes. For statistical analysis, generalized linear models (GLM) were adjusted and means compared by the Tukey test. The medium and ultrarapid velocity curves showed higher and equivalent values for total motility, progressive motility, and linear velocity of sperm (p<0.0...

Los antibióticos son utilizados en la criopreservación de semen equino con el fin de controlar la proliferación de microorganismos; sin embargo, estos pueden tener efectos negativos sobre los espermatozoides. La presente investigación tuvo como objetivo evaluar el efecto de dos preparaciones antibióticas sobre el desarrollo microbiano y la calidad de semen equino criopreservado. El semen de 10 caballos criollos colombianos fue criopreservado por congelación convencional, con dos preparaciones antibióticas: P1 (penicilina 10000 UI/ml y estreptomicina 10 mg/ml) y P2 (penicilina 10000 UI/ml, estreptomicina 10 mg/ml y anfotericina B 25 µg/ml) y un grupo control sin antibióticos. Posterior a la descongelación, se realizó la evaluación computarizada de la movilidad espermática, la prueba hipoosmótica (HOS) y el conteo de bacterias y hongos en el semen. El análisis estadístico s...

Antibiotics are used in cryopreservation of stallion semen in order to control the growth of microorganisms; however they can have negative effects on the spermatozoa. This study aimed to evaluate the effect of two antibiotic preparations on microbial growth and quality of cryopreserved stallion semen. Semen from 10 Colombian Creole horses was cryopreserved by conventional freezing with two antibiotic preparations: P1 (penicillin 10000 UI/ml and streptomycin 10 mg/ml) and P2 (penicillin 10000 UI/ml, streptomycin 10 mg/ml, and amphotericin B 25 μg/ml) and a control group without antibiotics. After thawing, computerized sperm motility evaluation, hypoosmotic test (HOS) and counting of bacteria and fungi in semen were performed. Statistical analysis was done using generalized linear models (GLM) and comparison of means by Tukey. Significant difference (p<0.05) for total motility (MT%) b...

La sabaleta (Brycon henni) es una especie de interés comercial en Colombia. Una de las principales barreras para los procesos de conservación de esta especie es la asincronía temporal entre los ciclos reproductivos de las hembras y los machos, que podría ser solucionado mediante el uso de procesos biotecnológicos. El objetivo de esta investigación fue evaluar la maduración in vitro de ovas de sabaleta (Brycon henni) mediante el uso de dos preparaciones hormonales. Un total de 1200 ovas fueron sometidas a maduración in vitro en presencia de extracto de hipófisis de carpa (EPC), hormona liberadora de gonadotropinas de salmón (sGnRHa) y un control sin estimulación hormonal. Las ovas se incubaron durante 2 horas a 25 °C en agitación orbital constante y 80% de O2. Se evaluó la maduración in vitro de las ovas mediante la clasificación de la posición del núcleo en cuatro categ...

The ‘sabaleta’ (Brycon henni) is a species of commercial interest in Colombia. One of the main barriers to the conservation processes of this species is the temporal asynchrony between the reproductive cycles of females and males, which could be solved using biotechnological processes. The aim of this investigation was to evaluate the in vitro maturation of ‘sabaleta’eggs (Brycon henni) using two hormonal preparations. A total of 1200 eggs were subjected to in vitro maturation in the presence of carp pituitary extract (EPC), salmon gonadotropin-releasing hormone (sGnRHa) and a control without hormonal stimulation. The eggs were incubated for 2 hours at 25 °C in constant orbital shaking and 80% O2. The in vitro maturation of the eggs was evaluated by classifying the position of the nucleus in four categories: migratory, central, atretic and peripheral. Higher maturation advanceme...