There is little previous information about the wild cycle of Toxoplasma gondii in wild carnivores and marsupials in Mexico. The objective of this study was to determine the presence of antibody against T. gondii in wild carnivores and marsupials in northeast Mexico. Frequency of T. gondii in captured specimens was: margay (Leopardus wiedii; n=3; 33%), jaguarundi (Puma yagouaroundi; n=2; 0%), bobcat (Lynx rufus; n= 1; 100%), coatimundi (Nasua narica; n=7; 42%), coyote (Canis latrans; n=4; 25%), gray fox (Urocyon cinereoargenteus; n=3; 66%), hognosed skunk (Conepatus leuconotus; n=1; 0%), and opossum (Didelphis sp.; n=5; 20%). Therefore, natural infection with T. gondii in wild carnivores and marsupials in northeast Mexico was present.