Los compuestos bioactivos presentes en plantas ornamentales va creciendo con el fin de combatir y prevenir distintas enfermedades, tanto en alimentos como en personas, por ello en esta investigación se realizó la extracción de bencil isotiocianato a partir de hojas de mastuerzo por diclorometano y CO2 supercrítico. Para ambas metodologías se usaron hojas liofilizadas a una humedad de 6% con módulo de finura 4,8. Para la extracción por diclorometano se usó hojas desengrasadas, se utilizó buffer fosfato de potasio pH 7 para acondicionar la hidrolisis y se purificó con columnas Resprep C18. Para la extracción con CO2 supercrítico, se trabajó a dos presiones (200 y 300 bar) y tres temperaturas (50, 60 y 70 °C). Se utilizó el patrón de bencil isotiocianato al 98% para la identificación y cuantificación del compuesto, que se realizó por cromatografía de capa fina y cromatog...

Tropaeolum majus L. is a native plant to South and Central America, used since ancient times by our ancestors to combat different diseases. Glucotropaeolonin is one of its main components, which when hydrolyzed forms benzyl isothiocyanate (BIT) that promotes cellular apoptosis (programmed cell death in cancer cells). Therefore, the present research aims to evaluate the effect of the pressure and temperature of benzyl isothiocyanate extraction by supercritical CO2 from Tropaeolum majus L. The extraction was carried out in a supercritical fluid extractor equipment Speed SFE BASIC Brand: Poly science, the leaves of Tropaeolum majus L. were ground for one hour and lyophilized until obtaining a humidity of 6%. The extraction with supercritical CO2 was carried out with pressures of 200 and 300 bar, temperatures of 50 °C, 60 °C and 70 C, obtaining by the conjugation of these six treatmen...

Tropaeolum majus L. is a native plant to South and Central America, used since ancient times by our ancestors to combat different diseases. Glucotropaeolonin is one of its main components, which when hydrolyzed forms benzyl isothiocyanate (BIT) that promotes cellular apoptosis (programmed cell death in cancer cells). Therefore, the present research aims to evaluate the effect of the pressure and temperature of benzyl isothiocyanate extraction by supercritical CO2 from Tropaeolum majus L. The extraction was carried out in a supercritical fluid extractor equipment Speed SFE BASIC Brand: Poly science, the leaves of Tropaeolum majus L. were ground for one hour and lyophilized until obtaining a humidity of 6%. The extraction with supercritical CO2 was carried out with pressures of 200 and 300 bar, temperatures of 50 °C, 60 °C and 70 C, obtaining by the conjugation of these six treatmen...

Tropaeolum majus L. is a native plant to South and Central America, used since ancient times by our ancestors to combat different diseases. Glucotropaeolonin is one of its main components, which when hydrolyzed forms benzyl isothiocyanate (BIT) that promotes cellular apoptosis (programmed cell death in cancer cells). Therefore, the present research aims to evaluate the effect of the pressure and temperature of benzyl isothiocyanate extraction by supercritical CO2 from Tropaeolum majus L. The extraction was carried out in a supercritical fluid extractor equipment Speed SFE BASIC Brand: Poly science, the leaves of Tropaeolum majus L. were ground for one hour and lyophilized until obtaining a humidity of 6%. The extraction with supercritical CO2 was carried out with pressures of 200 and 300 bar, temperatures of 50 °C, 60 °C and 70 C, obtaining by the conjugation of these six treatmen...

Tropaeolum majus L. is a native plant to South and Central America, used since ancient times by our ancestors to combat different diseases. Glucotropaeolonin is one of its main components, which when hydrolyzed forms benzyl isothiocyanate (BIT) that promotes cellular apoptosis (programmed cell death in cancer cells). Therefore, the present research aims to evaluate the effect of the pressure and temperature of benzyl isothiocyanate extraction by supercritical CO2 from Tropaeolum majus L. The extraction was carried out in a supercritical fluid extractor equipment Speed SFE BASIC Brand: Poly science, the leaves of Tropaeolum majus L. were ground for one hour and lyophilized until obtaining a humidity of 6%. The extraction with supercritical CO2 was carried out with pressures of 200 and 300 bar, temperatures of 50 °C, 60 °C and 70 C, obtaining by the conjugation of these six treatmen...