A guide to the echolocation pulses of 42 bat species recorded at eight stations near the Vilcanota‑Urubamba River, Cusco region, is presented. The objectives included optimizing the collection of clean, species‑specific acoustic recordings and developing an acoustic library in Peru. The flight tunnel is proposed as a more effective technique than the flight box, as it allows sustained flight in a single direction and the capture of high‑quality pulses. No defined taxonomic relationships based on echolocation pulses were identified. The variation in pulse shape was explained by the variables peak frequency and bandwidth, according to the PCA analysis. Cluster analysis formed four acoustic groups with no clear relationship to taxonomic, evolutionary, or ecological traits. Feeding acoustic libraries such as "Mashu Peru" will be crucial to more accurately describe the echolocatio...

En el presente trabajo de investigación, se evaluó la diversidad y bioacústica de murciélagos (Chiroptera), mediante ocho puntos de muestreo a lo largo de la cuenca del río Vilcanota desde el 2020 hasta el 2022, abarcando diferentes coberturas vegetales y gradientes altitudinales mediante redes de neblina y bioacústica en las provincias de Calca, Urubamba y La Convención. Se registraron 42 especies de murciélagos de las familias Phyllostomidae, Vespertilionidae y Emballonuridae, con un esfuerzo de muestreo de 2160 horas-red-día por punto. Carollia perspicillata fue la especie más abundante mediante captura con redes de neblina. La bioacústica detectó familias adicionales de Molossidae, Vespertilionidae, Emballonuridae y Phyllostomidae. El análisis de disimilaridad mostró dos grupos de comunidades divididas por la cordillera, aunque algunas especies como Anoura peruana y Stu...

 A guide to the echolocation pulses of 42 bat species recorded at eight stations near the Vilcanota‑Urubamba River, Cusco region, is presented. The objectives included optimizing the collection of clean, species‑specific acoustic recordings and developing an acoustic library in Peru. The flight tunnel is proposed as a more effective technique than the flight box, as it allows sustained flight in a single direction and the capture of high‑quality pulses. No defined taxonomic relationships based on echolocation pulses were identified. The variation in pulse shape was explained by the variables peak frequency and bandwidth, according to the PCA analysis. Cluster analysis formed four acoustic groups with no clear relationship to taxonomic, evolutionary, or ecological traits. Feeding acoustic libraries such as "Mashu Peru" will be crucial to more accurately describe the echolocatio...