El cáncer de mama (CM) es una de las neoplasias más frecuentes y una de las principales causas de mortalidad femenina a nivel mundial. A pesar de los avances en su diagnóstico y tratamiento, su etiología sigue siendo compleja y multifactorial. Recientes estudios sugieren un posible rol del Virus del Papiloma Humano (VPH) en la carcinogénesis mamaria, particularmente los genotipos de alto riesgo (16, 18 y 31), aunque su relación aún no está claramente establecida. El presente estudio tuvo como objetivo determinar la prevalencia de los tipos de VPH 16, 18 y 31 en muestras de tejido mamario maligno y benigno de pacientes atendidas en el Hospital Santa Rosa (Lima) entre 2021 y 2023. Se realizó un estudio transversal en 75 muestras parafinadas (FFPE), las cuales fueron sometidas a extracción de ADN mediante el kit EasyPure FFPE Tissue Genomic DNA y analizadas mediante PCR en tiempo ...

Quinoa (Chenopodium quinoa) is a highly nutritious crop that is resistant to adverse conditions. Due to the considerable increase in its commercial production in Andean soils, the plant is suffering the negative effects of monocropping, which reduces its yield. We used for the first time a high-throughput Illumina MiSeq sequencing approach to explore the composition, diversity, and functions of fungal and bacterial communities of the bulk and rhizosphere in soils of native C. quinoa affected by monocropping in the central Andes of Peru. The results showed that the bacterial and fungal community structure among the treatments was significantly changed by the monocropping and the types of soil (rhizosphere and bulk). Also, in soils subjected to monocropping, there was an increase in Actinobacteria and a decrease in Proteobacteria, and the reduction in the presence of Ascomycota and the inc...

Quinoa (Chenopodium quinoa) is a highly nutritious crop that is resistant to adverse conditions. Due to the considerable increase in its commercial production in Andean soils, the plant is suffering the negative effects of monocropping, which reduces its yield. We used for the first time a high-throughput Illumina MiSeq sequencing approach to explore the composition, diversity, and functions of fungal and bacterial communities of the bulk and rhizosphere in soils of native C. quinoa affected by monocropping in the central Andes of Peru. The results showed that the bacterial and fungal community structure among the treatments was significantly changed by the monocropping and the types of soil (rhizosphere and bulk). Also, in soils subjected to monocropping, there was an increase in Actinobacteria and a decrease in Proteobacteria, and the reduction in the presence of Ascomycota and the inc...

The characterization of soil microbial communities at different depths is essential to understand their impact on nutrient availability, soil fertility, plant growth and stress tolerance. We analyzed the microbial community at three depths (3 cm, 12 cm, and 30 cm) in the native fruit trees Annona cherimola (cherimoya) and Pouteria lucuma (lucuma), which provide fruits in vitamins, minerals, and antioxidants. We used PacBio-HiFi, a long-read high-throughput sequencing to explore the composition, diversity and putative functionality of rhizosphere bacterial communities at different soil depths. Bacterial diversity, encompassing various phyla, families, and genera, changed with depth. Notable differences were observed in the alpha diversity indices, especially the Shannon index. Beta diversity also varied based on plant type and depth. In cherimoya soils, positive correlations with Total Or...