The methylotrophic yeast Pichia pastoris is widely used as a eukaryotic system for expressing recombinant proteins. Over 500 recombinant proteins were expressed by P. pastoris with expression levels reaching up to 80% of total secreted proteins and up to 30% of total cell protein. The production process consists of a growth phase with glycerol and then an induction phase with methanol. Control culture conditions (pH, temperature, concentration of inducer, carbon and nitrogen source, etc.) are important for the growth of yeast to the production of the protein factors. On the other hand, malaria is a disease of great importance to global public health because of it is high prevalence mainly in tropical countries. In recent years there has been a breakthrough in the research of vaccines against Plasmodium species within the major antigenic proteins are the Antigen Membrane Apical (AMA) and ...

The methylotrophic yeast Pichia pastoris is widely used as a eukaryotic system for expressing recombinant proteins. Over 500 recombinant proteins were expressed in P. pastoris with expression levels reaching up to 80% of total secreted proteins and up to 30% total cell proteins. There are three phenotypes of P. pastoris classified according to their ability to metabolize methanol, phenotype MutS grows slowly on media containing methanol at generally low concentrations of methanol and longer induction time are used. Therefore, the control of culture conditions such as concentration of the inducer and the induction time are important factors for both yeast growth and for production of the protein since this system is controlled by the AOX promoter induced with methanol. Furthermore, L-asparaginase (EC. 3.5.1.1) is an important biopharmaceutical used to treat acute lymphoblastic leukemia (A...