Seed quality is affected by fungal contamination. These cause abortion, rot, or necrosis of the seed, as well as seedling damage that results in the development of diseases in later stages of growth. Their identification and characterization are essential to establish appropriate management. The objective of this work was to identify and determine the prevalence of fungal contaminants present in the seed of thirteen plant varieties of rice, soybean, corn, and forage sweet sorghum produced in the municipality of El Espinal, Tolima, Colombia. This research was carried out at the Tibaitatá Research Center of the Colombian Agricultural Research Corporation AGROSAVIA (Cundinamarca, Colombia) during the second semester of 2021. By means of a blotter test, fungal contaminants and their prevalence in germinated and non-germinated seeds were identified. A total of 65 fungal isolates were obtaine...