Stevia rebaudiana Bertoni (Bertoni) is a high-value source of non-caloric sweeteners, yet its genotype-specific performance under tropical highland conditions remains insufficiently documented. Eleven elite stevia clones grown in the tropical highlands of Indonesia were evaluated, integrating agronomic performance with the steviol glycoside profile. A random block design with four replicates was implemented across three harvests. Majalengka exhibited the greatest plant height (45.06 cm) and total biomass (21.32 g plant⁻¹), while Karang Anyar and Garut also exceeded 20 g plant⁻¹, indicating robust vegetative growth. Dry leaf yield ranged from 4.98 to 14.00 g plant⁻¹, with Majalengka, Karang Anyar, and TIA-001A among the highest-yielding genotypes. High-performance liquid chromatography (HPLC) identified Garut as the leading clone in total glycoside content (346.78 mg g⁻¹), wit...