This work was executed in the Plant Tissue Culture Laboratory at the National University of San Martín - Tarapoto. Its objective was assay five different culture media for in vitro gennination and development In Peruvian orchids Cattleya spp. and Oncidium lanceanum, they were sown from seeds using the green pod method and the statistical used was an ANOVA In a completely randomized design with five treatments and ten repeats each. Seeds started germination and protocom development 30 days after sowing in a germination culture media, with a very low levels of contamination, recorded after two weeks after sowing, because the aseptic conditions at replating from germination to development and rooting culture media. 24 weeks after sowing in culture medium for development and rooting (Phase 11), plantlets developed, in average values: 04 leaves for Cattleya spp. and 4,5 leaves for Oncidium l...