The objective of this study was to determine the seroprevalence of bovine brucellosis and identify risk factors for brucellosis in Pinar del Río province, Cuba. A cross-sectional study was performed based on the results of the epidemiological surveillance programme. Geospatial distribution was analyzed by calculating the standardized incidence ratio (SIR) at the municipality level. In addition, the association between diagnostic results and factors such as the productive system, sex and age groups (<12, 12-24, 24-36, >36 months) was assessed. A total of 12 760 blood samples were processed and 113 were positive; thus, the estimated seroprevalence in the province was 0.89% (95 % CI 0.74-1.06). The disease was present in three out of eight municipalities in the province (Los Palacios, Consolación del Sur and Pinar del Río), although only in Los Palacios the prevalence was sign...

A cross-sectional epidemiological study was carried out to determine animal and herd risk factors associated to bovine brucellosis. It was randomly selected 2369 cattle in the Manabí province, Ecuador. In addition, an epidemiological study of ecological type was carried out using 20 positive herds (cases) and 20 negative herds (controls) to the disease. In both studies, an epidemiological survey was conducted to determine the main risk factors associated to the disease. The individual study showed a higher risk of having the disease in animals older than 5 years (p<0.0001) and in females (p<0.05). Herds involved in milk production (p<0.05), those that do not check the aniamls before entering the premises (p<0.05) and those without vaccination (p<0.001) had a higher risk of being affected by the disease. It was also demonstrated that there was a greater probability of havi...

Se realizó un estudio epidemiológico transversal para determinar los factores de riesgo de los animales y de los hatos asociados a brucelosis bovina. Se seleccionaron 2369 bovinos de la provincia Manabí, Ecuador; asimismo, se realizó un estudio epidemiológico de tipo ecológico donde se seleccionaron 20 hatos positivos (casos) y 20 hatos negativos (controles) a brucelosis. En ambos estudios se aplicó una encuesta epidemiológica para determinar los principales factores de riesgo asociados a la presentación de la enfermedad. El estudio individual determinó un mayor riesgo de padecer la enfermedad para los animales mayores de 5 años (p<0.0001) y para hembras (p<0.05). En los hatos, las ganaderías dedicadas a la producción de leche (p<0.05) y las que no vacunan (p<0.001) presentan un mayor riesgo de afectarse por la enfermedad. Se comprobó, además, una mayor probabi...

A cross-sectional epidemiological investigation was carried out to determine the risk factors associated with the dissemination of brucellosis to humans in milk processing centres and slaughterhouses in seven cantons of the province of Manabí, Ecuador, between September and November 2015. A census was conducted to quantify farms processing milk, milk processing centres and slaughterhouses. A total of 119 dairy farms, seven slaughterhouses and seven milk processing centres were selected. They were characterized in terms of the types of products produced and on the presence of risk factors as causes of brucellosis in humans. The presence of antibodies against Brucella spp was determined in raw milk by the milk ring test. Results showed that 97.6% of the units make a single product (artisanal cheese). The presence of 85.0% of risk factor was found in milk processing dairies, value higher t...

Se realizó una investigación epidemiológica transversal para determinar los factores de riesgo asociados a la diseminación de brucelosis a los humanos en centro procesadores de leche y mataderos de siete cantones de la provincia Manabí, Ecuador, entre septiembre y noviembre de 2015. Se realizó un censo para determinar las unidades que elaboraban subproductos lácteos y los mataderos. Se seleccionaron 119 lecherías dedicadas a la elaboración de productos lácteos artesanalmente, siete mataderos y siete centros procesadores de productos lácteos, y se caracterizaron en cuanto a los tipos de productos que elaboran y la presencia de factores de riesgo como causales de brucelosis en humanos. Se determinó la presencia de anticuerpos contra Brucella spp en la leche utilizada como materia prima mediante la prueba del anillo. El 97.6% de las unidades elaboran un solo producto (queso arte...

The aim of this study was to determine the possible relationship between the presence of lesions called “White Spotted Kidney” (WSK) and the seroprevalence of Leptospira spp in cattle slaughtered in the Manta slaughterhouse, Manabí province, Ecuador. Blood samples were taken from 320 cattle to detect antibodies against Leptospira spp using the microagglutination technique. Likewise, a macroscopic revision of the kidneys was made, and samples were taken for histopathological analysis. The seroprevalence of Leptospira spp was 50.9% and the frequency of kidney injury was 18.13%. The presence of the eight serovars evaluated was found, with the serovars Pomona, Icterohaemorragiae and Bratislava being the most frequent. Animals serologically positive for Leptospira are 40.56 more likely to have specific lesions associated with the disease at the time of slaughter (p<0.00001; OR = 40.56...

The aim of this study was to determine the possible relationship between the presence of lesions called “White Spotted Kidney” (WSK) and the seroprevalence of Leptospira spp in cattle slaughtered in the Manta slaughterhouse, Manabí province, Ecuador. Blood samples were taken from 320 cattle to detect antibodies against Leptospira spp using the microagglutination technique. Likewise, a macroscopic revision of the kidneys was made, and samples were taken for histopathological analysis. The seroprevalence of Leptospira spp was 50.9% and the frequency of kidney injury was 18.13%. The presence of the eight serovars evaluated was found, with the serovars Pomona, Icterohaemorragiae and Bratislava being the most frequent. Animals serologically positive for Leptospira are 40.56 more likely to have specific lesions associated with the disease at the time of slaughter (p<0.00001; OR = 40.56...