The backscatter coefficient (BSC) is a fundamental property of tissues and can be used to classify tissues. Two BSC calibration methods are the planar reflector method and the reference phantom method. In both methods, linear acoustic propagation is assumed. In this study, the calibration methods were evaluated when acoustic nonlinear distortion was present. Radio frequency data were acquired from two physical phantoms using a 5 MHz single-element transducer and low power (one excitation level) and high power (six increasing excitation levels) excitation signals. BSCs estimated from the high power settings were compared to the BSCs estimated using the low power by calculating the root mean square error (RMSE). The BSCs were parameterized by fitting the BSC curve to a power law and estimating the power law exponent and by estimating the effective scatterer diameter (ESD). When using the p...

The ultrasonic attenuation coefficient (ACE) can be used to classify tissue state. Pulse-echo spectral-based attenuation estimation techniques, such as the spectral-log-difference method (SLD), account for beam diffraction effects using a reference phantom having a sound speed close to the sound speed of the sample. Methods like SLD assume linear propagation of ultrasound and do not account for potential acoustic nonlinear distortion of the backscattered power spectra in both sample and reference. In this study, the ACE of a sample was computed and compared using the SLD with two independent references (high attenuating and low attenuating phantoms but with similar B/A values) and over several pressure levels. Both numerical and physical tissue-mimicking phantoms were used in the study. The results indicated that the biases in ACE increased when using a reference having low attenuation, ...