Bacillus sphaericus 2362 liquid formulation, activity against Anopheles pseudopunctipennis and Culex quinquefasciatus larvae was tested in laboratory bioassays. Breeding place water and distilled water were used with concentrations of 1 x 10 11, 1,5 x 10 11, 2,5 x 10 11, 3,9 x 10 11 spores/mL. Target population was kept in laboratory conditions by using breeding native water and flora. One mL doses were added into test cups containing 25 II and III stage larvae and 150 mL of breeding water or distilled respectively; cups tests with the same amount of water and larvae were used as controls for each concentration, water type and larval specie tested. Testing was made three times for each concentration in laboratory conditions. Twelve replicas were made and divided in six for each species, using a total of 4800 larvae for species. Reading of larvae mortality were at 12, 24, 48, and 72 hours...

Bacillus sphaericus 2362 liquid formulation, activity against Anopheles pseudopunctipennis and Culex quinquefasciatus larvae was tested in laboratory bioassays. Breeding place water and distilled water were used with concentrations of 1 x 10 11, 1,5 x 10 11, 2,5 x 10 11, 3,9 x 10 11 spores/mL. Target population was kept in laboratory conditions by using breeding native water and flora. One mL doses were added into test cups containing 25 II and III stage larvae and 150 mL of breeding water or distilled respectively; cups tests with the same amount of water and larvae were used as controls for each concentration, water type and larval specie tested. Testing was made three times for each concentration in laboratory conditions. Twelve replicas were made and divided in six for each species, using a total of 4800 larvae for species. Reading of larvae mortality were at 12, 24, 48, and 72 hours...

Seven bioassays in laboratory were carried out in order to evaluate the biocidal capacity of Lonchocarpus utilis (Smith,1930) «barbasco» on 7000 larvae of third and fourth stage of Anopheles benarrochi (Gabaldon,1941), main vector of malaria in Yurimaguas and Loreto. The biocidal activity was probed with two types of quality water. Fresh roots of L. utilis were processed by Vílchez (1993) methodology, it was obtained a fined powder which contains as active ingredient the rotenone. The biocidal activity was measured with 5 doses of powder of the root diluted in distilled water: 6,25; 3,1; 2,1 ; 1,0 and 0,15 g/L. It was used 1 mL of the homogenized as inoculum for dose. Readings were made hourly until the 24 hours post treatment to determine the effectiveness and susceptibility. At the 12 hours post-treatment the doses of 6,25 and 3,1 g/L, obtained 98 and 89% of larval mortality when it...

Seven bioassays in laboratory were carried out in order to evaluate the biocidal capacity of Lonchocarpus utilis (Smith,1930) «barbasco» on 7000 larvae of third and fourth stage of Anopheles benarrochi (Gabaldon,1941), main vector of malaria in Yurimaguas and Loreto. The biocidal activity was probed with two types of quality water. Fresh roots of L. utilis were processed by Vílchez (1993) methodology, it was obtained a fined powder which contains as active ingredient the rotenone. The biocidal activity was measured with 5 doses of powder of the root diluted in distilled water: 6,25; 3,1; 2,1 ; 1,0 and 0,15 g/L. It was used 1 mL of the homogenized as inoculum for dose. Readings were made hourly until the 24 hours post treatment to determine the effectiveness and susceptibility. At the 12 hours post-treatment the doses of 6,25 and 3,1 g/L, obtained 98 and 89% of larval mortality when it...