Universidad Nacional Agraria La Molina. Facultad de Agronomía. Departamento Académico de Fitotecnia

In vitro micropropagation of pitahaya has been presented as an alternative for obtaining high quality plant material and mass production in reduced and controlled spaces. The present study aimed to achieve in vitro regeneration of shoots and roots of H. guatemalensis from areoles and their survival during ex vitro acclimatization. Different treatments with culture media, growth regulators and substrates were evaluated to obtain plants with normal development. Shoot regeneration from areoles was achieved 14 days, enriching the Murashige and Skoog (MS) medium with different concentrations of benzylaminopurine (BAP). The induction of shoots without callus formation and with greater shoot length and diameter was achieved using Woody Plant Medium (WPM) without cytokinins. On the other hand, root induction began at 14 days, obtaining between 90 and 100% of rooted explants, with the highest num...