Se evaluaron dos pruebas de campo para determinar la Mastitis Subclinica en bovinos. Se utilizó un total de 268 vacas en producción de leche, entre 1 a 10 partos, en dos sectores San Lorenzo y Miraflores de Levanto. En un sistema extensivo, su alimentación a base de pasturas naturales y cultivadas, un solo ordeño manual, y se consideró desde la segunda semana hasta 5 meses de lactación. Se utilizó el método biológico (Prueba California - Mastitis Test) y el método químico (Prueba de Whiteside), los que permitieron comprobar cuál de las pruebas fue más eficiente para la detección de la mastitis bovina. Los resultados demostraron que el 100 % de prevalencia de mastitis subclínica, según el número de partos, se reporta que el mayor porcentaje de prevalencia se muestra en el 4 parto y según los cuartos mamarios, se reporta que el anterior y posterior derecho reporta el 30 %...

Se evaluaron dos pruebas de campo para determinar la Mastitis Subclinica en bovinos. Se utilizó un total de 268 vacas en producción de leche, entre 1 a 10 partos, en dos sectores San Lorenzo y Miraflores de Levanto. En un sistema extensivo, su alimentación a base de pasturas naturales y cultivadas, un solo ordeño manual, y se consideró desde la segunda semana hasta 5 meses de lactación. Se utilizó el método biológico (Prueba California - Mastitis Test) y el método químico (Prueba de Whiteside), los que permitieron comprobar cuál de las pruebas fue más eficiente para la detección de la mastitis bovina. Los resultados demostraron que el 100 % de prevalencia de mastitis subclínica, según el número de partos, se reporta que el mayor porcentaje de prevalencia se muestra en el 4 parto y según los cuartos mamarios, se reporta que el anterior y posterior derecho reporta el 30 %...

The aim of this study was to evaluate the effect of inactivated feeder cells (feeder layer system) from two segments of the bovine oviduct (isthmus and ampulla) in the in vitro development of bovine embryos. Cell lines from segments of the isthmus and ampulla were generated and subsequently inactivated with mitomycin C (40 μg/ml) to inhibit their ability to divide and eliminate competition for nutrients with embryos. Bovine oocytes were matured in vitro for 24 h and were fertilized for 18 h in conventional culture with Brangus bovine semen. The expected fertilized oocytes were cultured for seven days in the feeder layer system with isthmus and ampulla cells separately at a concentration of 1.44 x 10 5 cells/ml. The best results in the production of in vitro bovine embryos were obtained with ampullary cells (280/84, 30%) in comparison with the isthmus cells (278/75, 26.9%) and the contro...

The aim of this study was to evaluate the effect of inactivated feeder cells (feeder layer system) from two segments of the bovine oviduct (isthmus and ampulla) in the in vitro development of bovine embryos. Cell lines from segments of the isthmus and ampulla were generated and subsequently inactivated with mitomycin C (40 μg/ml) to inhibit their ability to divide and eliminate competition for nutrients with embryos. Bovine oocytes were matured in vitro for 24 h and were fertilized for 18 h in conventional culture with Brangus bovine semen. The expected fertilized oocytes were cultured for seven days in the feeder layer system with isthmus and ampulla cells separately at a concentration of 1.44 x 10 5 cells/ml. The best results in the production of in vitro bovine embryos were obtained with ampullary cells (280/84, 30%) in comparison with the isthmus cells (278/75, 26.9%) and the contro...

OBJECTIVE This study aimed to report the impact of production practices and sanitary management on the prevalence of Neospora caninum (N caninum) and bluetongue virus (BTV) in Creole goats from the tropical dry forest of Utcubamba, Peru. METHODS 354 blood samples were obtained from male and female goats of diverse ages reared under an extensive productive system. The diagnosis of N caninum and BTV was conducted through a commercial ELISA kit (iD.vet) with readings taken on a Bio-Rad iMark microplate reader at 450 nm. Additionally, a survey was conducted with 18 goat producers to categorize them based on social, technical (production, health, reproduction, nutrition, and infrastructure), environmental, and economic factors. RESULTS The overall seroprevalence of N caninum and BTV in goats was 6.21% and 7.06%, respectively, Notably, group II farmers had the highest prevalence rates at 7.69%...