Staphylococcus aureus is a major cause of subclinical mastitis in dairy cows, and the development of antibiotic resistance has limited effective treatment options. This comprehensive study evaluated the virulence, antimicrobial resistance, and therapeutic alternatives based on essential oils (EOs) for spa type t605 S. aureus isolates from subclinical bovine mastitis in São Paulo, Brazil. The study also aimed to investigate the presence of virulence genes, identify genetic variations through spa and agr typing, determine phenotypic antibiotic resistance profiles, identify major chemical constituents of eight different EOs (Citrus aurantium bergamia - Bergamot, Copaifera reticulata - Copaiba, Foeniculum vulgare - Fennel, Zingiber officinale - Ginger, Ocimum basilicum - Basil, Luma chequeen – Arrayan, Mentha spicata - Spearmint, and Eugenia caryophyllus – Clove), and assess their antim...