In this work, we studied the degree of tolerance to heavy metals of fungi and bacteria isolated from soils with and without rhizosphere, in order to know its potential for applications in bioremediation. The samples came from Santa Rosa de Jangas mining liability. The fungi and bacterial strains were taxonomically identified by ITS region and 16S rDNA analysis, respectively. Heavy metal tolerance indices were calculate using salt medium supplemented with 1mM of lead (II), cupper (II), nickel (II) or zinc (II); and 0.1 mM of silver (I), chromium(VI) or cadmium (II). It was isolated 23 fungi and 18 bacteria strains. The fungi with better tolerance indices were Fusarium temperatum CTLM05 (Pb+2), Fusarium temperatum CTLM08 (Zn+2), Fusarium oxysporum CTLM18 (Ni+2 and Cd+2), Fusarium oxysporum CTLM12 (Ag+1), Fusarium inflexum CTLM22 (Cu+2), and Penicillium vanluykii CTLM11 (Cr+6). Likewise, th...

In this work, we studied the degree of tolerance to heavy metals of fungi and bacteria isolated from soils with and without rhizosphere, in order to know its potential for applications in bioremediation. The samples came from Santa Rosa de Jangas mining liability. The fungi and bacterial strains were taxonomically identified by ITS region and 16S rDNA analysis, respectively. Heavy metal tolerance indices were calculate using salt medium supplemented with 1mM of lead (II), cupper (II), nickel (II) or zinc (II); and 0.1 mM of silver (I), chromium(VI) or cadmium (II). It was isolated 23 fungi and 18 bacteria strains. The fungi with better tolerance indices were Fusarium temperatum CTLM05 (Pb+2), Fusarium temperatum CTLM08 (Zn+2), Fusarium oxysporum CTLM18 (Ni+2 and Cd+2), Fusarium oxysporum CTLM12 (Ag+1), Fusarium inflexum CTLM22 (Cu+2), and Penicillium vanluykii CTLM11 (Cr+6). Likewise, th...

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La contaminación del suelo por metales pesados se ha convertido en una amenaza para el ecosistema y la salud humana por su toxicidad y persistencia en la cadena alimenticia; así mismo inciden sobre la microflora promoviendo el desarrollo de una variedad de mecanismos metabólicos de tolerancia y acumulación, lo cual es favorable al ambiente. En este sentido el objetivo del presente trabajo fue caracterizar hongos y bacterias cultivables tolerantes a metales pesados del Pasivo ambiental minero Santa Rosa de Jangas. Se colectaron cuatro muestras de suelo de los cuales se aislaron hongos y bacterias mediante la metodología de dilución. Para la identificación taxonómica se analizó la reg ión ITS (hongos) y 16S rDNA (bacterias). Luego se evaluó el índice de tolerancia a Pb (11 ), Cu (11 ), Ni (11} y Zn (11 ) a 1mM y Ag (1), Cr (VI) y Cd (11) a 0.1 mM. En las cepas con mejores índi...