With the aim of developing a methodology of acclimatization of seedlings of pine nut (Jatropha curcas L.) cultivated in vitro from Zygotic embryos, studied 3 (1, 2 and 3 ppm) concentrations of two inductors of growth (AIB and ANA) and 3 levels of electrical conductivity of a solution of plant fertilizer (1,6; 2,2 and 2,8 mmhos) in pine seedlings propagated in vitro of 28 days in an atmosphere of incubation with a dimmable 10,76-2 734 lux, temperature 17,9 – 25,17 ° C, relative humidity of 30,2 – 41,7%, and a photoperiod of 16 hours light and 8 hours of darkness, with 30 days of acclimatization in an atmosphere of 21,71 pre – 36,57 º C temperature, 38,1 – 99,8% of relative humidity and 10,76 – 1 862 lux of light intensity, using a block with factorial arrangement design. The percentage of survival of seedlings in the phase of acclimatization this influenced by growth inducers ...