Bothrops atrox is the main venomous snake in the Amazonic region of Peru, because it is responsible of the majority of human ophidic accidents. For this reason, variation into chemical composition as well as enzymatic activities on this venom were studied in relation to age, considering 3 groups of captivity snakes: young of 1 and 2 years respectively as well as adult, major of 5 years. The venom obtained from the three groups was pooled, Iyophilized and properly dissolved into saline solution. Protein concentration at 280 nm and by Lowry's method was calculated into each venom sample. Besides, protein bands were determined by PAGE-SIDS. In addition, the following enzymatic activities were determined: caseinolytic, Amidolytic on BAPNA, coagulant, hialuroniclase, L-aminoacidoxidase (LAO) and fosfolipase A 2-Biological effects such as hemorrhage and edema were assayed on white mice. The re...

Bothrops atrox is the main venomous snake in the Amazonic region of Peru, because it is responsible of the majority of human ophidic accidents. For this reason, variation into chemical composition as well as enzymatic activities on this venom were studied in relation to age, considering 3 groups of captivity snakes: young of 1 and 2 years respectively as well as adult, major of 5 years. The venom obtained from the three groups was pooled, Iyophilized and properly dissolved into saline solution. Protein concentration at 280 nm and by Lowry's method was calculated into each venom sample. Besides, protein bands were determined by PAGE-SIDS. In addition, the following enzymatic activities were determined: caseinolytic, Amidolytic on BAPNA, coagulant, hialuroniclase, L-aminoacidoxidase (LAO) and fosfolipase A 2-Biological effects such as hemorrhage and edema were assayed on white mice. The re...