1
artículo
Publicado 2023
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The aim of this study was to update the epidemiological information of brucellosis in the province of Formosa, Argentina, based on records from the Research and Transfer Centre during the 2018-2021 period. In total, 151 218 samples of bovine sera from 1953 establishments in the province of Formosa were serologically analysed. Data analysis was done using logistic regression. The presence of spatial association clusters for populations with heterogeneous distribution was evaluated by scanning a data group less than 50% of the population at risk. The distribution of herds positive for brucellosis was assumed as a Bernoulli distribution. The prevalence of bovine brucellosis was 0.88%, while that of herds positive for brucellosis was 17.1%. The intra-herd prevalence was 5.73% (95% CI 0.34-24.18%). In 2018, a prevalence of 0.44% was observed with increases in 2019 (1.77%) and 2021 (0.52%). T...
2
artículo
Publicado 2022
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Immunoprophylaxis for bovine babesiosis has two presentations of a live attenuated vaccine (fresh and deep-frozen). Despite being effective, their durability, as well as the conditions necessary for their transfer and handling, make them impractical. The incorporation of a third presentation, using dehydrated merozoites as immunogens, constitutes an innovative alternative, which combines practicality and stability over time. The objective of this work was to test dehydration processes and cryopreservative and rehydrating substances. For this, erythrocytes highly parasitized with Babesia bovis and B. bigemina were subjected to two dehydration techniques: lyophilization and spray drying. In turn, for the lyophilization process, several lyoprotectants were tested: Dimethylsulfoxide, Glycerol, Dextrose and Polyvinylpyrrolidone (PVP). The only one that achieved a properly dehydrated powder, c...