The Peruvian Paso Horse (CPP) is a breed with a four-stroke gait originating in Peru from the horses that arrived at the time of the conquest of America. The aim of this study was to know the level of inbreeding and the effect on genetic variability in the breed. In total, 8330 animals were analysed that included the population of CPP born in Ecuador and their Peruvian ancestry (PT) and two reference populations of animals born in Ecuador (PE) and born between 2008 and 2018 (PR). The average traced generations were 9.18 ± 3.89 (PT), 11.14 ± 2.23 (PE) and 12.83 ± 1.42 (PR) and the completeness of the pedigree was over 90% in the first, fourth and fifth generations of PT, PE and PR, respectively. Average generation interval of 8.72 ± 4.56 (PT), 8.79 ± 4.30 (PE) and 9.20 ± 4.61 (PR). Average individual consanguinity: 5.97% (PT), 6.83% (PE) and 8.11% (PR) and kinship relationship: 9.02...