En el presente trabajo se evaluaron las alteraciones cariométricas causadas por la infección con la cepa Munantá, la cepa Tulahuén y el clon Dm28c de Trypanosoma cruzi en el tejido cardiaco de ratones ACA experimentalmente infectados con 2000 tripomastigotes sanguíneos del parásito y su relación con el daño inflamatorio, número de seudoquistes y posible asociación con los niveles de parasitemia y mortalidad acumulada. Los resultados mostraron un aumento significativo del tamaño nuclear en el tejido cardiaco de los ratones infectados en comparación con los ratones controles no infectados con T. cruzi. Los ratones infectados con la cepa Tulahuén o con el clon Dm28c mostraron una prepatencia sanguínea de cinco días y nivel máximo de parasitemia de 16.23 x 105 ± 2.21 x 105 parásitos/ml a los 11 días post infección – p.i. – (cepa Tulahuén) y de 20.10 x 105 ± 2.25 x 1...

This study aimed to evaluate the damage in the myocardial tissue in ovariectomized and non-ovariectomized Balb/c mice and the relationship with inflammatory damage, number of pseudocysts and correlation with parasitemia levels and mortality after experimental infection with 2000 blood trypomastigotes from clone Dm28c of Trypanosoma cruzi. Results showed a 3-day blood prepatency and higher parasitemia levels (p<0.001) during the first 9 days of infection (p.i.) in ovariectomized females when compared with non-ovariectomized females which showed a 5-day prepatency. However, the maximum parasitemia level reached by the ovariectomized females (18.09 x 104 ± 1.66 x 104 parasites/ml) at 11 days p.i. was lower (p<0.05) than the maximum parasitemia level of 26.03 x 104 ± 1.83 x 104 parasites/ml, observed in the non-ovariectomized females at 11 days p.i. The histopathological analysis sho...

En el presente trabajo se evaluaron las alteraciones cariométricas causadas por la infección con la cepa Munantá, la cepa Tulahuén y el clon Dm28c de Trypanosoma cruzi en el tejido cardiaco de ratones ACA experimentalmente infectados con 2000 tripomastigotes sanguíneos del parásito y su relación con el daño inflamatorio, número de seudoquistes y posible asociación con los niveles de parasitemia y mortalidad acumulada. Los resultados mostraron un aumento significativo del tamaño nuclear en el tejido cardiaco de los ratones infectados en comparación con los ratones controles no infectados con T. cruzi. Los ratones infectados con la cepa Tulahuén o con el clon Dm28c mostraron una prepatencia sanguínea de cinco días y nivel máximo de parasitemia de 16.23 x 105 ± 2.21 x 105 parásitos/ml a los 11 días post infección – p.i. – (cepa Tulahuén) y de 20.10 x 105 ± 2.25 x 1...

This study aimed to evaluate the damage in the myocardial tissue in ovariectomized and non-ovariectomized Balb/c mice and the relationship with inflammatory damage, number of pseudocysts and correlation with parasitemia levels and mortality after experimental infection with 2000 blood trypomastigotes from clone Dm28c of Trypanosoma cruzi. Results showed a 3-day blood prepatency and higher parasitemia levels (p<0.001) during the first 9 days of infection (p.i.) in ovariectomized females when compared with non-ovariectomized females which showed a 5-day prepatency. However, the maximum parasitemia level reached by the ovariectomized females (18.09 x 104 ± 1.66 x 104 parasites/ml) at 11 days p.i. was lower (p<0.05) than the maximum parasitemia level of 26.03 x 104 ± 1.83 x 104 parasites/ml, observed in the non-ovariectomized females at 11 days p.i. The histopathological analysis sho...

The aim of this study was to diagnose the presence of Piscirickettsia salmonis in samples of coho salmon (Oncorhynchus kisutch) from fish farms in the Los Lagos Region, Chile, which presented outbreaks of piscirickettsiosis. In total, 19 fish with clinical signs of piscirickettsiosis were used. Smears of kidney, liver and splenic tissue and intestinal content were made, and processed using the indirect immunofluorescence test (IFAT) for the detection of P. salmonis through a commercial kit. The data obtained were subjected to the Cochran and McNemar Q test in the Chi square distribution. Higher positivity was found in the liver (68.4%) and kidney (52.6%). In addition, the bacterium was found in the faeces (47.4%), indicating that the elimination via the faeces of the agent is possible under farming conditions. By associating the kidney and liver results, only 78.9% of the fish positive f...

The aim of this study was to diagnose the presence of Piscirickettsia salmonis in samples of coho salmon (Oncorhynchus kisutch) from fish farms in the Los Lagos Region, Chile, which presented outbreaks of piscirickettsiosis. In total, 19 fish with clinical signs of piscirickettsiosis were used. Smears of kidney, liver and splenic tissue and intestinal content were made, and processed using the indirect immunofluorescence test (IFAT) for the detection of P. salmonis through a commercial kit. The data obtained were subjected to the Cochran and McNemar Q test in the Chi square distribution. Higher positivity was found in the liver (68.4%) and kidney (52.6%). In addition, the bacterium was found in the faeces (47.4%), indicating that the elimination via the faeces of the agent is possible under farming conditions. By associating the kidney and liver results, only 78.9% of the fish positive f...