Was made an experimental study with longitudinal design to develop a qualitative and quantitative method to determine misoprostol ester through its metabolite misoprostol acid in serum samples. An ultraperformance liquid chromatograph equipment with a mass-mass detector (UPLC MS-MS) was used, obtaining peaks at 2,2 minutes of chromatographic running and concentrations between 3,1 and 18,4 ppb.

Se llevó a cabo un estudio experimental de diseño longitudinal con el objetivo de desarrollar un método cuali-cuantitativo para la determinación de misoprostol éster a través de su metabolito misoprostol ácido en muestras de suero. Se empleó un Cromatógrafo Líquido de Ultraperformance con detector Masa-Masa (UPLC MS-MS), obteniéndose picos a los 2,2 minutos de desarrollo cromatográfico del misoprostol ácido y concentraciones que se encuentran entre 3,1 y 18,4 ppb.

This study compared the organic carbon (OC) content in fractions of humic acids (HA) and fulvic acids (FA) in five soil orders (Aridisol, Entisol, Histosol, Inceptisol and Mollisol) and know their association with the degree of humification. Extraction and fractionation, as well as the degree of humification was carried out by the Nagoya method proposed by Kumada. OC quantification was determined by the Walkley and Black method. The results are: the average OC content of HAs of the order Aridisol differs from that of Histosols, Inceptisols, and Mollisols. The order Entisol presented differences with the Histosols and Mollisols, the soils of the order Inceptisol presented differences with the Aridisols and Histosols and those of the order Histosol differed from the Aridisols, Entisols and Inceptisols. Similarly, those of the Mollisol order differed with the Aridisols, Entisols and Incepti...

Humic substances (HS) are the main component of soil organic matter (SOM), a product of the pedogenetic process. In this study, the morphometric factors and climatic variable that condition the degree of humification, the organic carbon content of humic acids (HA) fulvic acids (FA) of 42 soil samples are related through the functional equation of factors of state of the soil proposed by Jenny. The degree of humification was determined by the Nagoya method proposed by Kumada. The quantification of organic carbon was determined using the method by Walkley and Black. The morphometric parameters of the relief were obtained from the Digital Elevation Model (DEM) and the climate parameter of the MODIS sensor. The results show that the relief factor conditions the degree of humification and the climate factor conditions the organic carbon content of humic acids (HA) and fulvic acids (FA).