Avian colibacillosis produced by Escherichia coli infections cause high mortality and morbidity in poultry farms. The objective of this study was to identify the pathogenic isolates of Peruvian broiler farms based in the presence of five genes encoding the virulence factors: iss, iucC, tsh, cvaC and irp2 by the Multiplex PCR. A total of 36 chicks between 14 and 31 days old from three poultry farms located in the north, centre, and south of Peru were sampled. Three colonies suspected to be E. coli were isolated from the intestine of each bird (n=108 colonies), and were biochemically tested with TSI, SIM, LIA, CITRATE and EMB mediums for the confirmation of the diagnostic. The 41 positive colonies to E. coli were analysed by Multiplex PCR, resulting 65.95% potentially pathogenic (carriers of two or more virulence genes); moreover, 9.8% of the isolates had all five genes. Three pathotypes c...

La colibacilosis aviar producida por infecciones de Escherichia coli es causa de elevados niveles de mortalidad y morbilidad en la industria avícola. El objetivo de este trabajo fue identificar las cepas patógenas de explotaciones avícolas en el Perú en base a la presencia de cinco genes codificadores de los factores de virulencia: iss, iucC, tsh, cvaC e irp2, mediante la técnica de Multiplex PCR. Se muestrearon 36 aves entre los 14 y 31 días de edad, provenientes de tres centros avícolas ubicados en el norte, centro y sur del país. Se hicieron aislados intestinales y se obtuvieron tres colonias sospechosas de E. coli por ave (n=108 colonias), que fueron evaluadas bioquímicamente con TSI, SIM, LIA y CITRATO, y adicionalmente con agar EMB para confirmación del diagnóstico. Las 41 colonias positivas a E. coli fueron analizadas mediante Multiplex PCR, resultando 65.9% de colonias...