The mechanisms of inflammation mediated by metabolites of lipoxygenase and nitric oxide play essential roles in physiological immune response. The aim of this study was to evaluate the lipoxygenase (LOX) inhibitory activity of methanolic extract of dried leaf of Cassia alata (Linn.) Roxb. as well as its effect on in vitro accumulation of nitric oxide (NO). The LOX inhibitory activity was evaluated for its ability to inhibit lipoxygenase (soyLOX) by absorbance-based assay using linoleic acid as the substrate while the influence of the extract on accumulation of NO was monitored spectrophotometrically by Griess assay method. Moreover, gas chromatographic analysis was carried out on the ether extract to identify and quantify the volatile compounds in the extract. The study showed effective soyLOX inhibitory activity correlating with the NO depletion in a concentration-dependent manner. The ...

The mechanisms of inflammation mediated by metabolites of lipoxygenase and nitric oxide play essential roles in physiological immune response. The aim of this study was to evaluate the lipoxygenase (LOX) inhibitory activity of methanolic extract of dried leaf of Cassia alata (Linn.) Roxb. as well as its effect on in vitro accumulation of nitric oxide (NO). The LOX inhibitory activity was evaluated for its ability to inhibit lipoxygenase (soyLOX) by absorbance-based assay using linoleic acid as the substrate while the influence of the extract on accumulation of NO was monitored spectrophotometrically by Griess assay method. Moreover, gas chromatographic analysis was carried out on the ether extract to identify and quantify the volatile compounds in the extract. The study showed effective soyLOX inhibitory activity correlating with the NO depletion in a concentration-dependent manner. The ...