Objective To determine the seroprevalence of infectious agents with reproductive implications in cattle from 3 districts of the high Andean region of Peru over 3,500 m above level sea. Methods 361 blood samples were collected from cows in 3 districts of the Apurimac department using nonprobabilistic stratified sampling for an exploratory study. Antibodies against Brucella spp, Mycobacterium avium subsp paratuberculosis, bovine viral diarrhea virus, bovine leukemia virus, bluetongue virus, bovine herpesvirus 1 (BHV), and Neospora caninum (NC) were detected by ELISA. Analysis of independence, multiple correspondence, and logistic regression were performed for risk factors, with district, age, and phenotypic traits as predictors. Results The highest seroprevalences were 18.28% BHV and 6.93% NC, with a similar trend at the sector and herd levels. Bovine herpesvirus 1 was predominant in Cotar...