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artículo
The aim of this study was to evaluate the potential for subchronic intoxication of Crotalaria pallida in sheep. Six rams were used, fed with hay and drinking water ad libitum, and 200 g/animal/day of commercial balanced ration. The sheep were distributed into a control group (G1) with two animals and an experimental group (G2) with four animals. In the G2 diet, 8 g of dry matter (DM) of C. pallida per kilogram of body weight were included for 28 days. All animals were clinically evaluated every 24 h. Animals were weighed and blood samples were taken for hemogram and liver and kidney profile on days 0, 14 and 28 post-exposure to C. pallida. All animals were slaughtered at the end of the study and samples were collected for histopathological analysis. No significant differences were found between groups with respect to clinical examination, hemogram data and blood chemistry; however, the h...
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artículo
The aim of the research was to carry out a systematic review about the epidemiology of SARS-CoV-2 infection in dogs and cats, as well as the genomic analysis of virus samples isolated from dogs and cats worldwide. For this, the systematic review was structured based on PRISMA’s protocol. Articles were obtained using the following keywords: SARS-CoV-2, COVID-19, dogs, cats, epidemiology, animal transmission, pets, companion animals, animal reservoirs and zoonosis. Additionally, all of SARS-CoV-2 genomes isolated from dogs and cats worldwide, reported in GISAID's EpiCoV™ database, were selected and analyzed through Nextclade’s tool for the generation of the respective phylogenetic trees. The exposure – natural infection with SARS-CoV-2 from January 2020 to October 2021 of 100 dogs and 108 cats positive by the RTq-PCR technique was reported worldwide. Furthermore, 141 SARS-CoV-2 gen...
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artículo
The aim of this study was to evaluate the sensitivity of four coprodiagnostic techniques to quantitatively determine the presence of Toxocara canis eggs in fresh canine stool samples. For this, eggs were extracted from the uterus of adult female T. canis obtained from canine puppies. Eight concentrations of eggs were used (10, 50, 75, 100, 250, 500, 750 and 1 000 eggs per gram of faeces - hpg), being considered as the gold standard to determine the degree of precision of the Kato-Katz, McMaster, Improved Modified McMaster and Faust techniques. Three repetitions were used per technique and per concentration. The sensitivity of each test was performed by arithmetic calculation and ROC curve (95% CI), comparing them with the gold standard by means of a nonparametric test of the Wilcoxon signed ranges (p<0.05). The Faust technique detected T. canis eggs regardless of concentration, but in...