Fasciola hepatica has a complex life cycle that includes a limnid snail as an intermediate host, where larval parthenogenetic multiplication occurs. We performed an experimental study of the life cycle of F. hepatica and estimated its potential biotic (r) in Galba truncatula. We selected 215 of these snails, and infected them with miracidia obtained at 26 ° C in the presence of light. We proceeded to dissect five snails every three days. By tracking the number of stages of F. hepatica by light microscopy observing external characteristics and development time of miracidia, sporocyst, rediae, and cercariae. We noted that the exponential growth model is best suited to the dynamics of F. hepatica. With a biotic potential of 6.69%, an average of 203 cercariae per miracidium and 270 cercariae per snail.

Fasciola hepatica is a digenean that has a complex life cycle which includes a vertebrate host in which its sexual reproduction occurs, verifiable in experimental animals. The objective of this study was to estimate the invasive power (PI), extension invasion (EI) and the biotic potential of this digenean in Rattus norvegicus albinus Holtzman strain, infected experimentally with F. hepatica's metacercariae which was obtained from infected snails in nature, in two provinces. Twenty metacercariae were administered in semisolid diet to six specimens of R. norvegicus, which were maintained in bioterio under controlled environmental conditions. We made coproparasitological analysis by simple concentration and sedimentation technique from the 86 to 100 days post infection and then proceeded with necropsies. We observed that the average production of eggs by adult F. hepatica was 9135.70 ± 219...

Galba truncatula snails were obtained from January to April 2011, from a sub urban biotope (Huayllapampa) district of San Jeronimo, Cusco, Peru, and considered hyperendemic. They were grown in an aquatic environment. They were experimentally infected (n= 215) with miracidia of Fasciola hepatica. The eggs were obtained from the gallbladder and bile ducts of three sheep with fasciolosis, screened and washed in boiled water cooled to incubate at a constant temperature of 26°C. The resulting collection of snails had an abundance of catch per unit effort of 150/h. Shells ranged between: 3.5 to 7.0 mm long, 2.2 to 3.6 mm in width. A survival 75.81%, of which 71.78% showed infection with F. hepatica was noted.

The development of eggs to obtain ciliated larvae called miracidia of Fasciola hepatica in the nature occur in aqueous biotopes or shallow banks of accumulations of water where the eggs come in fecal waste. The objective of this research was to make the hatching of miracidia exposed to light and darkness from eggs of F. hepatica from gall bladders of cattle and sheep hosts, and whether there are significant differences in the time of their evolution. The samples from 5 cattle and 10 sheep were collected from July to September 2009. Sieved and washed in cooled boiled water, placing 20 cm3 of sediment in each petri dish in triplicate and a constant temperature of 26°C and were observed daily until hatching, adding cooled boiled water to compensate the evaporation. Developing longer corresponded to the eggs incubated in darkness, being of 404 h and 439 h on eggs of origin sheep and cattle,...

Fasciola hepática tiene un ciclo biológico complejo, que incluye un caracol limneido como intermediario, donde acontece su multiplicación larval partenogenética, y un hospedador definitivo en el que acontece su reproducción sexual. El objetivo fue realizar un estudio experimental del ciclo de F. hepática, en un limneido y Rattus norvegicus, con un método alternativo para la mantención de limneidos de infección inducida, estimando el potencial biótico (r), la potencia invasiva (PI), extensión de la invasión (El) de F. hepática, y determinar la eficacia de Chaetogaster limnaei como su controlador natural en condiciones controladas de laboratorio. Los caracoles para este estudio fueron colectados los meses de diciembre del 2010 a abril del 2011, identificados por morfometría de sus conchas, y mantenidos en el laboratorio. Los huevos de F. hepática se obtuvieron del contenido ...