Objective: Identify the most frequent bacteria in diabetic foot infections and their sensitivity to antibiotics in patients of Belén Hospital of Trujillo between 2012 and 2016. Material and methods: Cross-sectional descriptive observational study. Medical records from patients diagnosed with diabetic foot that contained the result of a culture of secretion of the lesion with its antibiogram were reviewed. Results: The culture reports of 47 patients with 57 isolated microorganisms were analyzed. Gram-negative (64%) represented by Escherichia coli were the most frequently microorganisms and Gram-positive (36%) were represented by Staphylococcus aureus. A 42.75% of enterobacteria were producers of extended spectrum beta-lactamase (ESBL) and a 62.5% of Staphylococcus aureus were methicillin-resistant. All these microorganisms predominated in Wagner’s grade IV and V lesions and PEDIS’ gr...