Conventional earthquake drills often fail to provide safe, realistic, and repeatable training for procedural knowledge. This limitation is particularly critical in high-density settings, such as university environments, where it is vital to be prepared in the event of such disasters. Virtual reality (VR) combined with serious games (SGs) provides an opportunity for users to experience and practice responses to such events within a controlled environment. This research describes the design, development, and validation of VR-based SG for earthquake preparedness. The primary objective was to assess the effectiveness of the SG in improving university students’ knowledge of the measures to be taken in the event of earthquakes. The secondary objective was to analyze the correlation between the level of presence experienced in the SG and the knowledge gain. A pre-post study was conducted usin...