The aim of this study was to determine the relationship between climatic conditions and the two-dimensional electrophoretic profiles (2D-PAGE) of seminal plasma from Romosinuano (RS) bulls raised in the Colombian Caribbean. Semen was collected from 10 RS bulls during the dry and rainy seasons and quality parameters such as sperm concentration, viability, rapid individual progressive movement (FIPM) and hypoosmotic swelling test (HOST) were evaluated. Seminal plasma proteins were separated by 2D-PAGE electrophoresis, and the relative amount of each spot was related to seminal quality. Seminal quality was higher in the dry season period where 117 spots were detected with molecular weights between 13 and 80 kDa and isoelectric points between 3.0 and 8.5. The relative amount of 26 and 30 spots showed a positive correlation with FIPM and HOST, respectively. The spots highly related to these p...

The aim of this study was to determine by SDS PAGE the protein profile of the seminal plasma of Romosinuano (ROMO) and Costeño con Cuernos (CCC) bulls and their relationship with sperm quality parameters in fresh and thawed semen. Three ejaculates per bull were collected by electro-ejaculate from 10 ROMO and 10 CCC sires. Viability (VF), mass motility (MMF), individual rapid progressive motility (MIRPF) and sperm concentration (CE) were determined in fresh semen. Seminal plasma (PS) was obtained by double centrifugation. Individual motility of rapid progressives (MIRPPC), progressivity (PRPC), rapid progressives (RPPC) and medium progressive (MPPC) were determined in thawed semen, in both cases using the CASA computerized analysis system. Additionally, viability (VPC) and membrane integrity were determined by the hypoosmotic test. Proteins were separated by polyacrylamide gel electropho...