Pyoderma is one of the most diagnosed skin diseases in canines. Among the agents most involved were Staphylococcus intermedius; however, in 2005 it was reclassified into three phenotypically similar species: S. intermedius, S. pseudintermedius and S. delphini, which cannot be identified by conventional biochemistry. Various studies report S. pseudintermedius as the most frequently isolated bacterial agent in pyoderma. Therefore, this study evaluated the presence of S. pseudintermedius using PCR-RFLP in 141 Staphylococcus sp isolates in the Laboratory of Bacteriology of the Faculty of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú from cases of canine pyoderma in the period 2016-2018, finding that 87.9% of Staphylococcus sp isolates have been identified as S. pseudintermedius and 12.1% as Staphylococcus sp.

Pyoderma is one of the most diagnosed skin diseases in canines. Among the agents most involved were Staphylococcus intermedius; however, in 2005 it was reclassified into three phenotypically similar species: S. intermedius, S. pseudintermedius and S. delphini, which cannot be identified by conventional biochemistry. Various studies report S. pseudintermedius as the most frequently isolated bacterial agent in pyoderma. Therefore, this study evaluated the presence of S. pseudintermedius using PCR-RFLP in 141 Staphylococcus sp isolates in the Laboratory of Bacteriology of the Faculty of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú from cases of canine pyoderma in the period 2016-2018, finding that 87.9% of Staphylococcus sp isolates have been identified as S. pseudintermedius and 12.1% as Staphylococcus sp.

The aim of this study was to molecularly and serologically identify Salmonella spp present in isolates from avian eggs, carcasses, and viscera. In total, 46 isolates of avian origin identified as Salmonella spp were evaluated through cultures and biochemical tests in the period 2012-2017 from various districts of Lima. These isolates are kept in the Bacteriology Laboratory stock of the Universidad Nacional Mayor de San Marcos. The strains were reactivated, and suspicious Salmonella spp colonies were reconfirmed by culturing on selective media. Subsequently, the PCR was performed on the suspicious samples as a method of genetic diagnosis of Salmonella. The invasiveness gene invA, a gene involved with the virulence of Salmonella, was detected. Serotyping was performed with polyvalent and monovalent antisera for serogroup and serovar in the Enteropathogens Laboratory of the National Institu...