Con la finalidad de mejorar la propagación in vivo de la especie forestal Cinchona officinalis L., se practicaron varias técnicas de propagación sexual y asexual. Para la propagación sexual por semillas se probaron tres tipos de sustratos más dos testigos: sustrato tierra, arena y turba en las proporciones 1:1:1; 2:1:1 y 1:1:2 más los testigos turba (100 %) y tierra (100 %). Para la propagación asexual se realizaron los siguientes ensayos: a) ensayo de enraizamiento de estacas, donde se probó el efecto de dos hormonas comerciales: Hormonagro 1 y Enraizador H.V; b) ensayo de enraizamiento de brotes, donde se probó el efecto de dos hormonas comerciales: Hormonagro 1 y Enraizador H.V; y c) ensayo de enraizamiento de acodos aéreos, donde se probó tres tipos de sustratos: tierra orgánica, musgo sphagnun y aserrín. El más alto porcentaje de germinación de semillas de Cinchona of...

Con la finalidad de mejorar la propagación in vivo de la especie forestal Cinchona officinalis L., se practicaron varias técnicas de propagación sexual y asexual. Para la propagación sexual por semillas se probaron tres tipos de sustratos más dos testigos: sustrato tierra, arena y turba en las proporciones 1:1:1; 2:1:1 y 1:1:2 más los testigos turba (100 %) y tierra (100 %). Para la propagación asexual se realizaron los siguientes ensayos: a) ensayo de enraizamiento de estacas, donde se probó el efecto de dos hormonas comerciales: Hormonagro 1 y Enraizador H.V; b) ensayo de enraizamiento de brotes, donde se probó el efecto de dos hormonas comerciales: Hormonagro 1 y Enraizador H.V; y c) ensayo de enraizamiento de acodos aéreos, donde se probó tres tipos de sustratos: tierra orgánica, musgo sphagnun y aserrín. El más alto porcentaje de germinación de semillas de Cinchona of...

Plant micropropagation is a useful tool for the conservation of threatened forest species with the use of in vitro culture techniques. The main objective was to study the in vitro propagation of Cinchona officinalis L from seeds. NaOCl was applied at different concentrations and immersion times for seed disinfection, medium MS (Murashige and Skoog) was supplemented with GA3 for seed germination and for in vitro multiplication phase was used the combination of auxin and cytokinins in different concentrations. The treatments with NaOCl at 50 % for 5, 10 and 15 min were those that obtained the lowest percentage of contamination; the highest percentage of germination in the seeds was obtained with 1 mg/l of GA3 and the best results in vitro multiplication were obtained with the combination of 0.2 NAA + 2.0 BAP. In vitro pr...

Plant micropropagation is a useful tool for the conservation of threatened forest species with the use of in vitro culture techniques. The main objective was to study the in vitro propagation of Cinchona officinalis L from seeds. NaOCl was applied at different concentrations and immersion times for seed disinfection, medium MS (Murashige and Skoog) was supplemented with GA3 for seed germination and for in vitro multiplication phase was used the combination of auxin and cytokinins in different concentrations. The treatments with NaOCl at 50 % for 5, 10 and 15 min were those that obtained the lowest percentage of contamination; the highest percentage of germination in the seeds was obtained with 1 mg/l of GA3 and the best results in vitro multiplication were obtained with the combination of 0.2 NAA + 2.0 BAP. In vitro pr...

Plant micropropagation is a useful tool for the conservation of threatened forest species with the use of in vitro culture techniques. The main objective was to study the in vitro propagation of Cinchona officinalis L from seeds. NaOCl was applied at different concentrations and immersion times for seed disinfection, medium MS (Murashige and Skoog) was supplemented with GA3 for seed germination and for in vitro multiplication phase was used the combination of auxin and cytokinins in different concentrations. The treatments with NaOCl at 50 % for 5, 10 and 15 min were those that obtained the lowest percentage of contamination; the highest percentage of germination in the seeds was obtained with 1 mg/l of GA3 and the best results in vitro multiplication were obtained with the combination of 0.2 NAA + 2.0 BAP. In vitro pr...