Objective: To evaluate the depth of the gingival sulcus after the use of retractor thread 00 for class V restorations. Materials and Methods: For the present investigation, 84 dental pieces were evaluated in 46 patients, both men and women who attended the Clinic of the University of the Americas. Probing and control were performed at 72 hours to determine if it affected the retractor thread at the level of the gingival sulcus. Results: 64.29% of the pieces evaluated showed a change, while 35.71% remained in the normal standard. Regarding the differentiation of gingival biotypes, a greater change in the measurement of the gingival sulcus was obtained in patients with a thin biotype with 64.81% of the pieces evaluated in relation to the gross biotype with 35.19%. In the present study, the existence of bleeding at the level of the gingival sulcus was also determined at the time of the dril...