This study was carried out in the Center of Research and Instruction in Embryo Transfer (CIETE), sponsored by a partnership between the National Agrarian University of La Molina (UNALM) and the Ministry of Agriculture (MINAG), located at the UNALM campus. The objective was to evaluate the fertility and in vitro production of embryos of four bulls from the National Semen Bank. This study used 1031 oocytes of quality A and B obtained from ovaries of the local slaughterhouse. The oocytes were matured in the TCM-199 medium. The Percoll gradient centrifuge method was used for spermatic separation; sperm capacitation and fertilization was done in the TALP-IVF + PHE medium; and embryos were cultured with the Sofaa medium containing 5% Fetal Bovine Serum (FBS). Embryos were evaluated on days 7, 8, and 9 after fertilization. There were no statistical differences among the four bulls, and the fert...

This study was carried out in the Center of Research and Instruction in Embryo Transfer (CIETE), sponsored by a partnership between the National Agrarian University of La Molina (UNALM) and the Ministry of Agriculture (MINAG), located at the UNALM campus. The objective was to evaluate the fertility and in vitro production of embryos of four bulls from the National Semen Bank. This study used 1031 oocytes of quality A and B obtained from ovaries of the local slaughterhouse. The oocytes were matured in the TCM-199 medium. The Percoll gradient centrifuge method was used for spermatic separation; sperm capacitation and fertilization was done in the TALP-IVF + PHE medium; and embryos were cultured with the Sofaa medium containing 5% Fetal Bovine Serum (FBS). Embryos were evaluated on days 7, 8, and 9 after fertilization. There were no statistical differences among the four bulls, and the fert...

Background: There is currently no gold standard biomaterial for the treatment of periodontal intrabony defects (PIDs). One of the current options is the use of platelet-rich fibrin (PRF). Objective: To determine the clinical effect of PRF in the treatment of PID through a systematic review and meta-analysis. Materials and Methods: A literature search was conducted up to February 2017 in the following biomedical databases: Pubmed, Embase, Scielo, Science Direct, SIGLE, LILACS and in the Cochrane Central Register of Clinical Trials. The selection criteria included: randomized clinical trials published in the last 5 years, reporting clinical effects (probing depth, clinical insertion level or gingival recession), with a follow-up time equal to or greater than 6 months, and sample size larger than or equal to 10 patients reporting the use of PRF as a treatment for PID. The methodological qua...