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tesis de grado
Publicado 2022
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The study of malaria in birds in these last two decades has been increasing, for this, rapid and low-cost protocols are being developed for the extraction of genomic DNA, amplification and screening, allowing the presence of these hemoparasites to be detected. The objective of this research was to compare genomic DNA isolation protocols and to identify birds parasitized by the Plasmodium, Haemoproteus and Leucocytozoon genera that cause avian malaria in the Loreto Region, for which the salt precipitation protocol was used and modified ( Salting-out) and the method of isolation of blood cells by density gradient according to the conditions of the collected sample, proceeding to amplify by means of the Polymerase Chain Reaction in real time (qPCR) obtaining as results that the salt precipitation protocol showed better results in terms of its concentration and quality of ratios compared to ...