Cape gooseberry (Physalis peruviana L.) is a fruit crop with increasing economic and functional relevance, yet limited research exists on weed management practices for this species. This study aimed to evaluate the agronomic, physiological, and phytochemical responses of P. peruviana to various pre- and post-emergence herbicides under greenhouse and field conditions. Two biotypes were used to assess selectivity and crop tolerance to thirteen post-emergence and two pre-emergence herbicides. Post-emergence trials revealed that chlorimuron, fomesafen, and the mixture atrazine + simazine significantly reduced plant height and caused high phytotoxicity, especially under field conditions. Conversely, quizalofop, clethodim, fluazifop, and clodinafop (ACCase inhibitors) showed excellent selectivity and maintained yield levels comparable to the control. Pre-emergence applications of S-metolachlor...