The study was conducted in the district of Tamburco (Abancay, Apurimac, Peru) with the aim of comparing interpretation criteria of California Mastitis Test (CMT) in the diagnosis of subclinical mastitis in cattle. Two hundred and nine cows from a population of 459 hand-milked cows belonging to 95 breeders were used. A total of 828 functional mammary quarters were evaluated and the main criteria for CMT interpretation were estimated. The prevalence of subclinical mastitis in cows was 72.3 and 65.6%, and in total mammary quarters was 48.7 and 42.3% with and without considering traces as positives respectively, and without statistical difference. The estimated prevalence in individual mammary quarters varied between 48.3 to 49.3% and depending of the mammary quarter between 40.0 to 45.5% with and without considering traces as positives respectively (p<0.05). More intense positive reactio...

The aim of this study was to determine the effect of adding antioxidant Tempol in the individual and progressive motility of alpaca sperm at cooling and after thawing. Previously, the vas deferens was deviated in three males with suitable semen parameters. Sperm were collected three times per week and pooled to avoid the individual effect. The sample was distributed in four aliquots and placed in refrigeration to reach 5 °C in 2 h. The antioxidant was added in four concentrations: 0, 0.5, 1.0 y 1.5 mM when the aliquots reached 10 °C. The sperm were filled in 0.25 ml straws, placed over liquid nitrogen vapor by 6 min and then submerged. Individual motility at cooling was 55.1, 63.7, 52.8 and 45.0 (p<0.05) and after thawing 35.7, 42.3, 29.0 and 18.1% (p<0.05) for 0, 0.5, 1.0 and 1.5 mM Tempol, respectively. Progressive motility at cooling was 36.0, 42.4, 28.4 and 21.7 (p<0.05)an...

The study was conducted in the district of Tamburco (Abancay, Apurimac, Peru) with the aim of comparing interpretation criteria of California Mastitis Test (CMT) in the diagnosis of subclinical mastitis in cattle. Two hundred and nine cows from a population of 459 hand-milked cows belonging to 95 breeders were used. A total of 828 functional mammary quarters were evaluated and the main criteria for CMT interpretation were estimated. The prevalence of subclinical mastitis in cows was 72.3 and 65.6%, and in total mammary quarters was 48.7 and 42.3% with and without considering traces as positives respectively, and without statistical difference. The estimated prevalence in individual mammary quarters varied between 48.3 to 49.3% and depending of the mammary quarter between 40.0 to 45.5% with and without considering traces as positives respectively (p<0.05). More intense positive reactio...

El presente estudio tuvo como objetivo determinar el efecto de la adición del antioxidante Tempol en la motilidad individual y progresiva a la refrigeración y descongelación de espermatozoides de alpaca. Se desvió el conducto deferente de tres alpacas con adecuados parámetros seminales y los espermatozoides fueron colectados tres veces por semana y mezclados para anular el efecto individual. La muestra colectiva se dividió en cuatro alícuotas y fueron colocadas en refrigeración hasta alcanzar 5 ºC en 2 h. El antioxidante fue añadido en concentraciones de 0, 0.5, 1.0 y 1.5 mM cuando las alícuotas alcanzaron los 10 ºC. Las muestras fueron envasadas en pajillas de 0.25 ml, colocadas sobre vapores de nitrógeno líquido por 6 min y sumergidas. La motilidad individual de refrigeración fue 55.1, 63.7, 52.8 y 45.0 (p<0.05) y a la descongelación 35.7, 42.3, 29.0 y 18.1% (p<0....

Universidad Nacional Agraria La Molina. Escuela de Posgrado. Doctorado en Ciencia Animal