Aisla un bacteriófago lítico denominado Vf1, procedente del mar peruano, capaz de infectar a Vibrio fluvialis, bacteria que afecta humanos y organismos acuáticos. El bacteriófago Vf1 fue caracterizado con respecto a su morfología, estabilidad térmica, diferentes rangos de pH, sensibilidad frente al cloroformo, exposición a la luz UV, rango de hospederos y la curva de crecimiento de un paso. La microscopía electrónica de transmisión, evidenció cabeza de forma icosaedrica con un diámetro de 82.773 nm y un talo largo no contráctil de 142.318 nm de longitud, características propias a la familia Siphoviridae. El fago Vf1, fue resistente al calor a 20°C por 30 minutos, perdiendo su viabilidad a partir de 50 y 60°C; y en valores de pH entre 7 y 8 mantuvo títulos elevados, disminuyendo el título entre 5 y 6, siendo nulo a valores de pH ácido. La viabilidad frente a la exposici...

Vibrio alginolyticus is associated with diseases in aquaculture. The misuse of antibiotics has led to the search for alternatives in the treatment of bacterial diseases, among them the application of bacteriophages that infect and destroy bacteria selectively. In this way, a highly lytic V. alginolyticus bacteriophage, termed Va1, was isolated, with the aim to evaluate its physical chemical parameters. For this purpose, different temperature, pH, chloroform exposure and host range conditions were evaluated. The temperature stability of phage Va1 showed higher titers at 20 and 30 °C decreasing from 40 °C. With respect to pH, the highest titers for the bacteriophage were between 5 and 8, and chloroform exposure reduced viability of the Va1 phage by 25%. The one-step curve determined that the latency period and the burst size were 20 minutes and 192 PFU / infective center respectively. Un...

Vibrio alginolyticus is associated with diseases in aquaculture. The misuse of antibiotics has led to the search for alternatives in the treatment of bacterial diseases, among them the application of bacteriophages that infect and destroy bacteria selectively. In this way, a highly lytic V. alginolyticus bacteriophage, termed Va1, was isolated, with the aim to evaluate its physical chemical parameters. For this purpose, different temperature, pH, chloroform exposure and host range conditions were evaluated. The temperature stability of phage Va1 showed higher titers at 20 and 30 °C decreasing from 40 °C. With respect to pH, the highest titers for the bacteriophage were between 5 and 8, and chloroform exposure reduced viability of the Va1 phage by 25%. The one-step curve determined that the latency period and the burst size were 20 minutes and 192 PFU / infective center respectively. Un...