In this research the biological control of Colletotrichum gloeosporioides, which causes anthracnose in crops of economic interest, was evaluated. The pathogen faced the following antagonistic microorganisms: Clonostachys rosea, Fusarium oxysporum and Trichoderma harzianum. Agar Sabouraud Dextrose (DSA) and Agar Papa Dextrosa (PDA) were used at different temperatures and in dual cultures. The percentage of inhibition, degree of antagonism and the rate of growth of the colonies were evaluated. It was found that the most effective antagonist was Trichoderma harzianum, which reached a degree 2 on the Bell scale (75% of the medium surface), in a culture medium Papa Dextrose Agar at 10°C 20°C and 30°C to 3, 6, 9 and 12 days of development. In addition, it was demonstrated that C. gloesporioides reached a degree 3 on the Bell scale (50% of the surface of the medium) when it faced C. rosea an...