Pharmacokinetic parameters of danofloxacin at 2.5% were determined after intravenous and intramuscular application in adult goat females. Plasma levels were quantified by HPLC with fluorescence detector set at 295 nm excitation and 490 nm emission using mobile phase composed of water, acetonitrile and triethylamine (79:19:1 v/v/v) adjusted to pH 3.0. In each route of application, using plasma concentrations versus time data in each animal, pharmacokinetic parameters were determined with PK Solution 2.0 software. Quantification limit of the assay was 0.0048 μg/ml. The kinetic profile exhibited by danofloxacin in goats was similar to that of fluoroquinolones in domestic animals. The intramuscular application provides rapid absorption, produces a Cmax of 0.36 ± 0.13 μg/ml at 55.1 ± 13.8 minutes, and exhibits wide distribution in the organism and bioavailability (F) of 109.7 ± 19.8%. Da...

Pharmacokinetic parameters of danofloxacin at 2.5% were determined after intravenous and intramuscular application in adult goat females. Plasma levels were quantified by HPLC with fluorescence detector set at 295 nm excitation and 490 nm emission using mobile phase composed of water, acetonitrile and triethylamine (79:19:1 v/v/v) adjusted to pH 3.0. In each route of application, using plasma concentrations versus time data in each animal, pharmacokinetic parameters were determined with PK Solution 2.0 software. Quantification limit of the assay was 0.0048 μg/ml. The kinetic profile exhibited by danofloxacin in goats was similar to that of fluoroquinolones in domestic animals. The intramuscular application provides rapid absorption, produces a Cmax of 0.36 ± 0.13 μg/ml at 55.1 ± 13.8 minutes, and exhibits wide distribution in the organism and bioavailability (F) of 109.7 ± 19.8%. Da...

The action of menthol on physiological and anesthesiological variables in rainbow trout was evaluated. Clinically healthy juvenile trout were used, of both sexes, formed in group A (n=20) of 201.4 ± 60.1 g of weight subjected to 100 ppm/L of menthol in water at pH 6.4 and in group B (n=20) of 187.3 ± 48.6 g of weight exposed to identical concentration of menthol with the water adjusted to pH 8.0 with sodium bicarbonate. The fish were placed in containers with water at 16.7 °C and electrical conductivity of 0.10 mS cm-1. The work methodology was: 1) acclimatization of fish in a container without drug and recording of the basal respiratory rate (RR) after adopting a normal swimming behavior; 2) transfer to a second container with menthol and recording of times of loss of lateral balance and anesthesia; 3) transfer of anesthetized fish to a third container without drug and record of RR a...

The aim of this study was to evaluate the response of eugenol, propofol, isoflurane and lidocaine on physiological and anaesthesiologic variables of Cheirodon interruptus, the most widely used baitfish in Argentina. Six groups were formed: 25 mg/l eugenol, 50 mg/l eugenol, 300 mg/l lidocaine, 0.2 mg/l isoflurane, 0.4 mg/l isoflurane, and 5 mg/l propofol. The tests are carried out with water at a temperature of 18.03±2.1 °C, pH 6.87±0.18, dissolved oxygen 6.73±0.05 mg l-1 and conductivity 3.74±0.04 μS cm-. The initial weight (1.6±0.4 g) and total length (5.1±0.4 cm) were statistically similar between groups. The results indicate that eugenol and isoflurane meet the requirements that a good anaesthetic for use in fish should meet. For propofol and lidocaine, it is considered necessary to review the dosage to adapt its use in C. interruptus due to the long recovery time. The fish tr...