In this paper, the characteristics of the physical-chemical degradation potential of soybean methyl biodiesel were studied, applied to samples of B100, B5, and diesel in storage tanks, including the analysis of microorganisms present in sample bodies with the correct corrosion measurement for AISI 1020 and 316L steel in different environments. Inside the tanks, immersion tests were performed on the sample body, maintaining storage conditions, dynamic conditions, and ambient temperature, for 120 days, collecting samples regularly to evaluate media degradation and analyze microorganisms to develop physicalchemical characterization, oxidation stability (OSI), and water content. Once the storage stage was completed, the corrosion ratio was measured in mm/ year according to ASTM D3707 and the presence of biological microorganisms.The oxidation stability of the B100 samples had ...