Trichinellosis is a global parasitic food-borne disease affecting humans, domestic and wild animals. Beyond its health implications, trichinellosis also has negative economic impacts for the pork industry. However, to date, few studies have analyzed Trichinella infections in the Amazon region. This study aimed to increase the information on the circulation of Trichinella spp. in free-ranging peccaries in two areas in the Northern Peruvian Amazon. Between 2008 and 2020, local subsistence hunters collected dried blood spots on filter paper from 140 free-ranging Pecari tajacu and 93 Tayassu pecari (n= 233 total), to test for antibodies against Trichinella spp. All animals were negative in serological tests for anti-Trichinella spp. antibodies. No evidence of nematodes was also found in the histological sections of the analyzed peccary diaphragm samples (n=29). In the two study areas, free-r...

Hepatitis B virus (HBV) and Hepatitis E virus (HEV) are zoonotic pathogens posing significant health concerns in rural Amazonia, a region marked by high endemicity, poverty, and limited healthcare access. However, the epidemiology of HBV and HEV in this ecosystem remains underexplored. This study examines the circulation of HBV and HEV at the human–wildlife interface and identifies risk factors within an isolated Amazonian indigenous community reliant on hunting for subsistence. Antibodies against HBV core antigens (HBcAbs) were found in three wildlife species: Cuniculus paca (0.8%), Tayassu pecari (1.6%), and Mazama americana (4.1%), marking the first record of HBV antibodies in free-ranging wildlife in the Amazon. However, further research is necessary to identify circulating strains and their relation to human HBV. HBcAbs were also detected in 9.1% of human samples, confirming expos...