Seed samples of wheat harvest in 2010 from Santo Augusto, Passo Fundo and Vacaria, RS; were plated on gerbox acrylic sterilized culture medium PSA (potato, sucrose and agar) plus antibiotics. Were used 400 seeds of each cultivar total of 45 cultivars. Were distributed equally spaced 25 seeds in each seedling with four replications, totaling 100 seeds per replicate. The seeds were incubated at 25 ± 2°C in an incubator with a photoperiod of 12 hours for 12 days. The experimental design was blocks and the experimental unit consists of four gerbox containing 25 seeds each. Was considered to be infected the seed of conidiophore presence and/or conidia of the fungus. The data were transformed to (x+1)1/2 and subjected to analysis of variance and means compared by Tukey test at 5% probability. Fungi F. graminearum and Alternaria spp. were detected in high incidence in most lots, performing at...

Seed samples of wheat harvest in 2010 from Santo Augusto, Passo Fundo and Vacaria, RS; were plated on gerbox acrylic sterilized culture medium PSA (potato, sucrose and agar) plus antibiotics. Were used 400 seeds of each cultivar total of 45 cultivars. Were distributed equally spaced 25 seeds in each seedling with four replications, totaling 100 seeds per replicate. The seeds were incubated at 25 ± 2°C in an incubator with a photoperiod of 12 hours for 12 days. The experimental design was blocks and the experimental unit consists of four gerbox containing 25 seeds each. Was considered to be infected the seed of conidiophore presence and/or conidia of the fungus. The data were transformed to (x+1)1/2 and subjected to analysis of variance and means compared by Tukey test at 5% probability. Fungi F. graminearum and Alternaria spp. were detected in high incidence in most lots, performing at...

This study aimed to identify and quantify the causal agents of fungal leaf blights, and compare different assessment methods for disease quantification. The experiments were conducted with 20 medium cycle cultivars in Passo Fundo, Saint Augustus and Vacaria counties with three replications in randomized blocks. For evaluation 20 leaves were collected per plot, at flowering stage. Disease severity was estimated by the percent diseased leaf area and lesions number per leaf. For fungus etiology, twenty five discs of each leaf sample containing a lesion were subjected to a humid chamber. After three days incubation fungi identification was performed under a dissecting microscope. The mean incidence, number of lesions and severity data were subjected to analysis of variance and means compared by the Tukey’s multiple test at 5% significance level. The severity and lesions number were the mos...

This study aimed to identify and quantify the causal agents of fungal leaf blights, and compare different assessment methods for disease quantification. The experiments were conducted with 20 medium cycle cultivars in Passo Fundo, Saint Augustus and Vacaria counties with three replications in randomized blocks. For evaluation 20 leaves were collected per plot, at flowering stage. Disease severity was estimated by the percent diseased leaf area and lesions number per leaf. For fungus etiology, twenty five discs of each leaf sample containing a lesion were subjected to a humid chamber. After three days incubation fungi identification was performed under a dissecting microscope. The mean incidence, number of lesions and severity data were subjected to analysis of variance and means compared by the Tukey’s multiple test at 5% significance level. The severity and lesions number were the mos...