The present study allows us to know how to assess the sequestration of CO2 in natural forest, in plots VII and VIII of the “Huayo” Arboretum of CIEFOR, Puerto Almendra, Iquitos, 2021, of the Faculty of Forestry Sciences of the National University of the Amazon. Peruvian, quantitative type and non-experimental design. In plot VII there is a total of 341 259,34 kg of biomass and 170,63 tC, and in plot VIII there is a total of 363 470,18 kg of biomass and 181,74 tnC. Plot VII sequesters 625,58 tnCO2, whose valuation is USD 51 189,42; and in plot VIII, it sequesters 666,30 tnCO2 whose valuation is USD 54 521,08. To determine the hypothesis, the Kolmogorov-Sminorv normality test was performed, which indicated that it does not have a normal distribution, therefore, the non-parametric Kruskal-Wallis test has a p-value of 0.035 lower than the level of significance of 0.05, indicating that th...