Cloning and protein expression in heterologous systems are very useful tools for the study of viral proteins. In this work, an in vivo cloning strategy was applied using the yeast Saccharomyces cerevisiae, as an efficient and low-cost method to clone several cDNAs from the tilapia lake virus (TiLV). Samples of infected tilapia Oreochromis niloticus tissues were taken and used to isolate their RNA and to obtain and clone the ten viral cDNAs in a shuttle plasmid. The cloning efficiencies range from 5 to 100% but for seven of the cDNAs the values were above 40%, demonstrating the high efficiency of the method. © 2021 The Authors. Journal of the World Aquaculture Society published by Wiley Periodicals LLC on behalf of World Aquaculture Society.

Acid mine drainage (AMD) is a major environmental issue due to its high heavy metal concentrations and low pH, posing risks to ecosystems and human health. In Hualgayoc, Cajamarca, Peru, AMD contamination from mining activities necessitates effective remediation strategies. This study evaluated the removal efficiency of organic amendments, municipal waste compost (MWC), cattle manure compost (CMC), vermicompost (HMS), corn stalk biochar (CSB), sludge biochar (SLB), pine biochar (PBC), and native macrophytes Carex pichinchensis (CAX), Juncus ecuadoriensis (JSP), and Myriophyllum aquaticum (MYA) in removing As, Cd, Cu, Fe, and Zn. A physicochemical characterization of AMD, amendments, and plants was conducted, and sorption capacity was determined through triplicate analyses. The results showed that SLB achieved a 100% Zn removal, while CSB removed over 90% of Cu and Fe. Among the organic a...