Cytogenetic biomonitoring techniques allow early detection of exposure to genotoxic agents and the sister chromatid exchange assay (SCE) that was applied aimed to evaluate these effects in peripheral blood lymphocytes of health personnel exposed to antineoplastic drugs in a Hospital Complex of Lima. The exposed group was subdivided according to the proximity to the oncological preparations in: direct, intermediate and low contact, in addition there was a control group. The information was analyzed in the statistical program IBM SPSS 25.0. Statistically significant differences were found in the frequencies of exchanges in the groups of health personnel with direct and intermediate exposure compared to the control group (P<0.05), the comparative analysis between personnel with leave and the control group did not show significant differences. These findings allow evidence of a higher ris...

The aim of this study is to induce specific antibody IgY in Gallus gallus immunized with membrane extracts of merozoites of Plasmodium falciparum. Three groups were formed from 2 hens each, the first group was inoculated intravenously 0.5 mL. membrane extracts of Plasmodium falciparum merozoites. , Another group with 1.0 mL. membrane extracts of Plasmodium falciparum merozoites., having also a control group who is 1.0mL inoculum. physiological saline. Eggs were collected daily and stored in refrigeration at 4 oC, we proceeded to work with eggs from 15 days post inoculation. Ig Y was purified with PBS deslipidación And - Chloroform and fractional protein precipitation with ammonium sulfate. We evaluated the immunoglobulin concentration (mg / ml) of egg yolk with the method of Brandford. Were analyzed by western blot, which revealed 2 bands with relative masses antigen between 42 and 19 k...

Aim: To determine the characteristics of suspected adverse reactions reported by health personnel of the hospital III – Chimbote. Method: We performed a descriptive, cross-sectional, retrospective, framed in the field of Hospital Pharmacovigilance, were analyzed and classified all spontaneous reports of suspected RAM given to the Pharmacological Committee of Hospital III - Chimbote, from 1 january to 31 december of 2008. Results and Conclusions: There were 65 reports of suspected RAM with a total of 110 drugs involved. The most representative drug classes were: antihypertensives (43.6%), antibiotics (22.8%), NSAIDs (10.9%). The 41.5% of notifications were made by doctors, 48.5% for pharmaceutical chemist, in the type of RAM these were mostly of type B, with respect to severity, 45.5% were non-serious, the rate 37.3% 15.5% serious and severe type, and about 67.3% causality was probable,...

Las técnicas citogenéticas de biomonitoreo permiten la detección temprana de la exposición a los agentes genotóxicos, para este estudio se utilizaron el ensayo cometa (EC) y el análisis de intercambio de cromátidas hermanas (ICH), el objetivo fue evaluar los posibles efectos en linfocitos de sangre periférica del personal sanitario expuesto a medicamentos antineoplásicos en un Complejo Hospitalario Policial de Lima. El grupo expuesto se dividió en 3 subgrupos, de acuerdo con la cercanía a los preparados oncológicos en: exposición directa, intermedia y baja, además se dispuso de un grupo de control. La información fue analizada en el programa estadístico IBM SPSS 25.0. Se hallaron diferencias estadísticamente significativas en los valores de ICH entre el personal sanitario con exposición directa e intermedia comparados con el grupo de control (p<0,05), el análisis compa...