Periodontal disease is characterized by the presence of complex subgingival microbial communities, encompassing up to 500 bacterial species, with Socransky’s red complex being particularly notable. However, recent systematic reviews have identified the critical role of other microorganisms in biofilm formation and dental surface colonization, including Staphylococcus aureus, Enterococcus faecalis, Helicobacter pylori, and Candida albicans, which may also act as significant pathogens within the periodontal context. This study aimed to analyze the presence of lesser-known microorganisms that could play a crucial role in periodontal disease. A descriptive documentary research approach was employed through a narrative bibliographic review, involving searches for scientific articles in databases such as Google Scholar, PubMed, SciELO, and Scopus. Thirty-four articles meeting the establis...

Objective: To determine the quality of life related to oral health in 18-99-year-old people in the city of Macas-Ecuador 2021. Materials and methods: This was a descriptive, cross-sectional study following a quantitative approach. A total of 415 people over 18 years of age from Macas-Ecuador were evaluated. The instrument of measure was a OHIP-14 instrument composed of 7 dimensions, each of which had 2 questions. The study variables (sex, age, socioeconomic level, occupation) were analyzed using the Mann-Whitney U statistical test, with p <0.05 being considered as significant. Results: There was a predominance of 63% of women. The dimension of “psychological distress” had the highest overall impact of all the items answered. Comparison of the OHIP-14 dimensional scores between men and women showed statistically significant differences in functional limitation and handicap with p =...

Objectives: The aim of this article was to investigate the pathogen Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and identify it by polymerase chain reaction (PCR). Materials and methods: The alkaline lysis technique was used for DNA extraction, and a segment of the 16S rRNA gene of A. actinomycetemcomitans was amplified by PCR and specific primers. The specificity of the primers was tested using genetic material extracted from the reference strain A. actinomycetemcomitans ATCC 33384, against other strains. Results: This pathogen was investigated in detail and A. actinomycetemcomitans was identified by means of specific primers. Conclusions: A sensitive and specific technique for the detection of A. actinomycetemcomitans was obtained by conventional PCR.

Objectives: The aim of this article was to investigate the pathogen Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and identify it by polymerase chain reaction (PCR). Materials and methods: The alkaline lysis technique was used for DNA extraction, and a segment of the 16S rRNA gene of A. actinomycetemcomitans was amplified by PCR and specific primers. The specificity of the primers was tested using genetic material extracted from the reference strain A. actinomycetemcomitans ATCC 33384, against other strains. Results: This pathogen was investigated in detail and A. actinomycetemcomitans was identified by means of specific primers. Conclusions: A sensitive and specific technique for the detection of A. actinomycetemcomitans was obtained by conventional PCR.